All I see in our family tree is a whole lot of dead.
The diagram above represents the main biological relationships to Sam and Dean.
- Dean Winchester - The eldest son of Mary and John, brother of Sam, half brother to Adam Milligan.
- Sam Winchester - The youngest son of Mary and John, brother of Dean, half brother to Adam Milligan.
- Mary Winchester (née Campbell) - Wife of John, mother of Sam and Dean. Daughter of Samuel and Deanna Campbell.
- John Winchester (deceased) - Husband of Mary, father of Sam, Dean and Adam Milligan; son of Henry Winchester.
- Kate Milligan (deceased) - Lover of John Winchester, mother of Adam Milligan.
- Adam Milligan (deceased) - Son of John Winchester and Kate Milligan, half brother to Sam and Dean.
- Emma (deceased) - Daughter of Dean and the Amazon Lydia.
- Henry Winchester (deceased) - Grandfather of Sam and Dean, and father of John Winchester who disappeared in 1958 only to arrive in 2013 and die at the hands of Abaddon. After Henry's disappearance in 1958, John may have been raised by a step-father, or grandfather/uncle, as his 'old man' in 1973.
- Millie Winchester (deceased) - Wife of Henry Winchester and mother of John Winchester.
- Bobby Singer (deceased) - Surrogate father figure to the boys from their childhood. He definitely considered Sam and Dean to be his sons - "As fate would have it, I adopted two boys. And they grew up great. They grew up heroes."
- Castiel - Dean tells Castiel that he is like a brother to him and Sam.
- Jessica Moore (deceased) - Sam's girlfriend while he was at Stanford. He was about to propose to her when Azazel had her killed by Brady, in order to force Sam back into hunting.
- Lisa Braeden and Ben Braeden - Dean had a brief affair with Lisa when he was young, and he looked her up when he was in his last year following his deal with the Crossroads Demon. Dean returned to them following Sam's sacrifice to imprison Lucifer. He lived with them for a year. Eventually, he asked Castiel to wipe him from their memories, due to the danger and violence he felt he brought to their lives.
- Amelia Richardson - Sam had a relationship with Amelia for a the better part of a year, while Dean was in Purgatory. They lived together in Kermit, Texas. The relationship ended when Amelia's husband Don Richardson, returned after having been presumed killed in action in Afghanistan, and Sam chose to return to hunting over staying with Amelia.
- Benny Lafitte (deceased) - After their time together in Purgatory, Benny and Dean have a close friendship, close enough that Benny calls Dean "brother," something he doesn't rebut and he trusts him to save Sam and Bobby. Benny never betrays Dean's trust and friendship.
- Charlie Bradbury (deceased) - An ally of the Winchester boys. Dean claims that "She's kind of like the little sister I never wanted." Even Charlie considered the Winchester boys her brothers.
- Kevin Tran (deceased) - Dean tells Kevin that after everything he's been through with them, Dean considers Kevin a member of the family and that each other, Sam and Castiel are all they have left in the world. Kevin apparently considers Dean the same as he stays when Dean tells him to leave if that means nothing to him.
- The Impala - Definitely a member of the family. Before moving into the Men of Letters Bunker, the car acted as Sam and Dean's home.
- Campbell (family) for more relatives on Mary's side of the family tree.