|Dates||???-2012 (killed by Sam)|
|Episode(s)||7.13 The Slice Girls|
Charlene is a detective in the Seattle Police Department and an Amazon.
In Seattle, the Amazons are breeding, leaving in their wake four dead men in two weeks with their hands and feet removed and an Amazonian symbol carved in their chest. Sam and Dean start investigating and meet Charlene Penn, the lead detective on the case.
However, Charlene is also an Amazon who informs Madeline that the Winchesters are on their trail. Madeline orders the death of Sam, along with Dean.
As Sam leaves a meeting with Professor Morrison, Detective Penn confronts and attacks him, but Sam kills her.