Castiel finds Crowley, and with the help of Jo Harvelle the brothers enter his house which is protected from angels by Enochian symbols. To their surprise he shoots his henchmen and gives them the Colt, stating that he wants Lucifer dead because he believes that eventually Lucifer will kill all demons after the humans are dead. Back at Bobby's place, Bobby takes a photo of himself, Sam, Dean, Ellen, Jo and Castiel, as he believes that this might be their last night on Earth. When the team arrives in Missouri, Castiel sees reapers everywhere. He tries to find out why they are there and is captured in a ring of flaming holy oil by Lucifer, who tries to convince Castiel to join him. Meg arrives and sends hellhounds after the brothers and the Harvelles, and while trying to help Dean, Jo is severely wounded. While taking shelter in a hardware store, Dean calls Bobby, who tells him the Reapers are in town because they are waiting for their boss to show: Death, one of the Four Horsemen. Realizing she won't recover from this, Jo suggests they build a bomb to blow up the hellhounds, using her as bait to lure the demonic dogs. Ellen volunteers to stay behind, too, so she can let the hellhounds in and set off the bomb while Dean and Sam escape. After a tearful goodbye with Dean, Jo dies in Ellen's arms, who then blows up the building, killing herself and the hellhounds.
The brothers find Lucifer having killed all the women and children in the town as part of the ritual to summon Death, and with all the men possessed by demons. Dean shoots Lucifer with the Colt. However, he recovers instantly and reveals that there are only five beings the Colt can't kill — and he happens to be one of them. Lucifer sacrifices several demons to summon Death, stating that "they're just demons." Meanwhile, Castiel manages to free himself from his trap by throwing Meg into the flames and walking over her, and takes the brothers to Bobby's. There, Bobby throws the picture showing them all into the fire as if it is a Hunter's funeral pyre and together with the brothers, grieves silently over Jo and Ellen's death.
Crowley: Your choice. You can cling to six decades of deep-seated homophobia, or give it up and get a complete bailout for your bank's ridiculous incompetence.
I want you to take this thing to Lucifer and empty it into his face.
Dean: Uh-huh, okay, and why exactly would you want the Devil dead?
Crowley: It's called -- survival. But I forgot -- you two, at best, are functional morons—
functioning... morons. Mor...
Dean: Oh, uh, excuse me for asking, but aren't you kind of signing your own death warrant? I mean, what happens to you if we go up against the Devil and lose?
Crowley: Number one, he's going to wipe us all out anyway. Two, after you leave here, I go on an extended vacation to all points nowhere. And three, how about you don't miss, okay! Morons!
It's gotta be a trap, right?
Dean: Sam Winchester, having trust issues with a demon. Well, better late than never.
Sam: Yeah, and thank you again for your continued support.
So. Dangerous mission tomorrow. Guess it's time to eat, drink, and, you know, make merry.
Jo: Are you giving me the last-night-on-earth speech?
Dean: No... If I was, would, uh, would that work?
Jo: No. Sweetheart, if this is our last night on Earth, then I'm going to spend it with a little thing I call self-respect.
If you're into that kinda thing.
So I take it you're here with the Winchesters.
Castiel: I came alone.
Lucifer: Loyalty. Such a nice quality to see in this day and age. Castiel, right? Castiel. I'm told you came here in an automobile.
Lucifer: What was that like?
Castiel: Um. Slow. Confining.
What a peculiar thing you are.
Lucifer: I rebelled, I was cast out. You rebelled, you were cast out. Almost all of Heaven wants to see me dead, and if they succeed, guess what? You're their new public enemy number one. We're on the same side, like it or not, so why not just serve your own best interests? Which in this case just happen to be mine?
Meg: Your call. You can make this easy or you can make it really, really hard.
Dean: When have you known us to ever make anything easy?
You got another plan? You got any other plan? Those are hellhounds out there, Dean. They've got all of our scents. Those bitches will never stop coming after you. We let the dogs in, you guys hit the roof, make a break for the building next over. I can wait here with my finger on the button, rip those mutts a new one. Or at least get you a few minutes' head start, anyway.
Ellen: No, I—I won't let you.
This is why we're here, right? If I can get us a shot on the devil—Dean, we have to take it.
Lucifer: I was a son. A brother, like you, a younger brother, and I had an older brother who I loved. Idolized, in fact. And one day I went to him and I begged him to stand with me, and Michael—Michael turned on me. Called me a freak. A monster. And then he beat me down. All because I was different. Because I had a mind of my own. Tell me something, Sam. Any of this sound familiar? Anyway. You'll have to excuse me. Midnight is calling and I have a ritual to finish. Don't go anywhere. Not that you could if you would.
"Abandon All Hope" is a reference to the final line of the inscription over the Gates of Hell in Dante's Inferno
- "Abandon all hope, ye who enter here."
The demon Crowley is making a deal; even as we speak, it's—going—down.
Dean: Going down? Right. Okay, Huggy Bear, just don't lose him.
- Huggy Bear was the name of the streetwise pimp who acted as an informant for Starksy and Hutch on the '70s TV show.
Yes. Um. Nick is wearing a bit thin, I'm afraid. He can't contain me forever, so—
- May be a reference to the line in the Doctor Who serial "The Tenth Planet" in which the First Doctor remarks that "this old body of mine is wearing a bit thin" prior to his first regeneration.
It sounds like Death, son. I think Satan's in town to work a ritual. I think he's planning to unleash Death.
Dean: You mean like, as in this dude and taxes are the only sure thing?
- This is a reference to Benjamin Franklin's famous quote, "In this world nothing can be said to be certain, except death and taxes."
Unleash? I mean, hasn't Death been tromping all over the place? Hell, I've died several times myself.
Bobby: Not this guy. This is—this is the angel of death. Big daddy reaper. They keep this guy chained in a box six hundred feet under. Last time they hauled him up, Noah was building a boat. That's why the place is crawling with reapers. They're waiting on the big boss to show.
- This is a reference to the story of Noah's Ark and the Biblical deluge, as told in the Book of Genesis.
And Dean. Kick it in the ass... don't miss.
- "Kick it in the ass" was a catchphrases of the late Kim Manners. Samantha Ferris related in her blog how the line came about:
The shout out to Kim Manners was an idea from one of the Camera guys, Brad. He came up to me quietly on the down low before we shot that piece where i say goodbye to Dean and said, "hey, when you turn around to say "don't miss" try one where you say "kick it in the ass" ". The line was just supposed to be "....and Dean, don't miss". We did both. They put both in. It was a bittersweet moment for the cast and crew. Robin the props gal cried......Kim is/was and will always be a very loved and missed man. Especially on that set.
You listen to me, you son of a bitch. I'm gonna kill you myself, you understand me? I'm going to rip your heart out!
- Sam telling Lucifer that he is going to rip his heart out may be a reference to The Prophecy In this movie the only way to kill an angel was to remove its heart.
We're gonna win. Can you feel it? You cloud-hopping pansies lost the whole damn universe. Lucifer's gonna take over heaven. We're going to Heaven, Clarence.
- A reference to It's a Wonderful Life, in which the angel Clarence shows Jimmy Stewart that life is worth living, and then finally gets his wings and goes to heaven.