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Twizzlers became a fandom icon following a CW Connect Interview with Jared on April 16, 2008.

During Jared's interview, as he is talking about planning a prank on Kim Manners Jensen walks in with a Twizzler in his mouth. He pokes Jared in the face with the Twizzler. Jared responds "Don’t Twizzler me." Jensen winks and smiles at the camera with the Twizzler still in his mouth, and then walks away.

As the interview was filmed during the season finale 3.16 No Rest for the Wicked, it is likely the red Twizzler came from the set. A jar of them can be seen on a table in the Fremont house during Lilith's 'birthday' party.