After the [[Mark of Cain]] causes him to get [[Rudy|another hunter]] killed, Dean summons Death in hopes Death can kill him. However, the Mark's power is such that even Death can't do that and he explains about [[the Darkness]] and the consequences of removing the Mark. He offers to send Dean somewhere far away where he is no threat, but demands Sam's life in exchange. While Dean initially agrees, he instead turns Death's [[Death's Scythe|own scythe]] on him, killing Death who crumbles to dust.
In 2017, its revealed that the [[reaper]] [[Billie]] has taken Death's place due to a rule stating that the next reaper to die after an incarnation of Death is killed becomes Death themselves.